Fig. 4. HOTAIR knockdown increases the mRNA levels of negative regulators of the Wnt/β-catenin signaling pathway maybe by demethylation of theirs promoters in HeLa cells. HeLa cells were transfected with DsiCTRL or DsiHOTAIR, after 48 (left) or 96 (right) hours, the mRNA level of PCDH10, SOX17, AJAP1 and MAGI2 genes was determined by RT-qPCR (A). Analysis of promoter methylation, after 48 (left) or 96 (right) by MSP. Below is shown the densitometry analysis (B). The bars represent the mean ± standard deviation from at least three independent experiments. *p˂0.05.